Prabhu, Chandan and Valechha, Aarti and Wanjari, Snehal and Labhsetwar, Nitin and Kotwal, Swati and Satyanarayana, T and Rayalu, Sadhana (2011) Carbon composite beads for immobilization of carbonic anhydrase. Journal of Molecular Catalysis B: Enzymatic, 71. pp. 71-78. ISSN 1381-1177

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Abstract

Fixation of anthropogenic CO2 into calcium carbonate using carbonic anhydrase (CA) as a bio-catalyst appears to be a promising option of CO2 sequestration. In the present study, carbonic anhydrase (CA) has been immobilized on chitosan based activated alumina–carbon composite beads. Synthesized adsorbent was thoroughly characterized using XRD, FTIR, SEM and BET-SA. Maximum adsorption capacities were determined using Langmuir model and it was observed to be 172.41 mg/g. Mechanistic and kinetic aspects have been addressed for immobilized as well as free enzyme. The Km and Vmax for immobilized enzyme was 10.35mM and 0.99�mol/ml/min, whereas for free enzyme it was 1.89mM and 0.99�mol/ml/min respectively. Proof of concept has been established for carbonation reaction. The carbonate has been characterized using various tools such as XRD and SEM which confirms the calcite nature of calcium carbonate. The CO2 sequestration capacity in terms of conversion of CO2 to carbonate was quantified by gas chromatography (GC). The CO2 sequestration capacity of immobilized beads was found to be 19.22mg of CaCO3/mg of enzyme as compared to 33.06mg of CaCO3/mg of enzyme for free enzyme.

Item Type:	Article
Uncontrolled Keywords:	Bio-Sequestration; Carbonic Anhydrase; Carbon Composite Beads Enzyme immobilization Carbonation
Subjects:	Materials Science
Divisions:	UNSPECIFIED
Depositing User:	Dr. Nitin Labhasetwar
Date Deposited:	10 Jan 2018 05:23
Last Modified:	10 Jan 2018 05:23
URI:	http://neeri.csircentral.net/id/eprint/1034

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