Volobaev, Valentin P. and Larionov, Aleksey V. and Kalyuzhnaya, Ekaterina E. and Serdyukova, Ekaterina S. and Yakovleva, Svetlana and Druzhinin, Vladimir G. and Babich, Olga O. and Hill, Elena G. and Semenihin, Victor A. and Panev, Nikolay I. and Minina, Varvara I. and Sivanesan, Saravana Devi and Naoghare, Pravin and Silva, Juliana da and Barcelos, Gustavo R. M. and Prosekov, Alexander Y. (2018) Associations of polymorphisms in the cytokine genes IL1β (rs16944), IL6 (rs1800795), IL12b (rs3212227) and growth factor VEGFA (rs2010963) with anthracosilicosis in coal miners in Russia and related genotoxic effects. Mutagenesis, 32 (2). pp. 129-135. ISSN 0267-8357, ESSN: 1464-3804

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Abstract

Anthracosilicosis (AS), a prevalent form of pneumoconiosis among coal miners, results from the accumulation of carbon and silica in the lungs from inhaled coal dust. This study investigated genotoxic effects and certain cytokine genes polymorphic variants in Russian coal miners with АS. Peripheral leukocytes were sampled from 129 patients with AS confirmed by X-ray and tissue biopsy and from 164 asymptomatic coal miners. Four single-nucleotide polymorphisms were genotyped in the extracted DNA samples: IL1β T-511C (rs16944), IL6 C-174G (rs1800795), IL12b A1188C (rs3212227) and VEGFA C634G (rs2010963). Genotoxic effects were assessed by the analysis of chromosome aberrations in cultured peripheral lymphocytes. The mean frequency of chromatid-type aberrations and chromosome-type aberrations, namely, chromatid-type breaks and dicentric chromosomes, was found to be higher in AS patients [3.70 (95% confidence interval {CI}, 3.29–4.10) and 0.28 (95% CI, 0.17–0.38)] compared to the control group [2.41 (95% CI, 2.00–2.82)and 0.09 (95% CI, 0.03–0.15)], respectively. IL1β gene T/T genotype (rs16944) was associated with AS [17.83% in AS patients against 4.35% in healthy donors, odds ratio = 4.77 (1.88–12.15), P < 0.01]. A significant increase in the level of certain chromosome interchanges among AS donors is of interest because such effects are typical for radiation damage and caused by acute oxidative stress. IL1β T allele probably may be considered as an AS susceptibility factor among coal miners.

Item Type:	Article
Uncontrolled Keywords:	Alleles; Oxidative Stress; Diagnostic Radiologic Examination; Roentgen Rays; Chromosome Abnormality; Lung; Polymorphism; Biopsy; Anthracosilicosis; Carbon; Chromatids; Chromosomes; Coal; Coal; Mining; DNA; Dust; Genes; Genotype; Growth Factor; Leukocytes; Lymphocytes; Pneumoconiosis; Single Nucleotide Polymorphism; Russia; Silicon Dioxide; Interleukin-6; Cytokine Gene; Il12b Gene; Donors
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Mr. RK Lohiya
Date Deposited:	08 Mar 2019 07:45
Last Modified:	08 Mar 2019 07:45
URI:	http://neeri.csircentral.net/id/eprint/1189

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