Saha, Shaswati and Vrieze, Jo De and Biswas, Rima and Nandy, Tapas (2018) In-situ ammonia removal by methanogenic granular biomass. Environmental Science: Water Research & Technology (4). pp. 559-568. ISSN 2053-1400

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Abstract

Methanogenic grannules from sewage-fed 298-day old UASB reactor having high abundance of Nitrospira sp. were evaluated for simultaneous methanogenesis and nitrification. Batch experiments were conducted under anaerobic and oxygen limited condition using either 0.04% (v/v) methanol (C) or ammonium chloride (30-100 mg N/L) (N) or both (N+C). Results showed that C-utilization preceded N-utilization in N+C sets indicating methanogenesis was independent of ammonia removal. Ammonia removal with nitrate generation under oxygen-limited condition confirmed nitrification potential of methanogenic grannule. However, under anaerobic conditions, decrease in ammonium nitrogen did not result in stoichiometric increase in nitrite & nitrate concentrations. Further, hydrogenotrophic methanogens disappeared in N & N+C sets. H2-mediated denitrification could possibly atrribute for disappearance of both nitrite or nitrate and hydrogenotrophic methanogens. Increase in Methanosaetaceae compensated for methane production via acetoclastic route. Thus, simultaneous nitrification & H2-mediated denitrification was confirmed as the plausible route for ammonia removal in methanogenic granules under anaerobic condition. This finding of in-situ ammonia removal in methanogens has opened new avenues for treating C/N wastewaters.

Item Type:	Article
Subjects:	Water Treatment Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Mr. RK Lohiya
Date Deposited:	26 Mar 2019 11:29
Last Modified:	26 Mar 2019 11:29
URI:	http://neeri.csircentral.net/id/eprint/1218

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