Thul, Sanjog and Nigam, Bhavna and Tiwari, Sarita and Pandey, R A (2019) Arsenite-oxidation performance of microbes from abandoned iron ore. Indian Journal of Biotechnology , 18 (1). pp. 34-41. ISSN 0975-0967

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Arsenite is considered to be more toxic than arsenate (an average of 100 times). It can be oxidized to arsenate by chemical reaction or microbiological interactions. The aim of the present study was to investigate the arsenite-oxidizing microbes that exist in iron ore mine in India. We cultured 13 morphologically distinct bacterial strains, among which, 6 strains could grow in high concentrations and shows the arsenite transforming abilities. Analysis of the amplified 16S rDNA gene sequences of the isolates revealed them to belong to alpha-, gamma- Proteobacteria and Firmicutes particularly in genera Paenibacillus, Pseudomonas, Ochrobactrum, Enterobacter and Bacillus. Further, qualitative silver nitrate screening assay and quantification by HPLC-ICP-MS analysis of these strains indicated the transformation of arsenite to arsenate. Moreover, the isolates were genetically analyzed for presence of arsenic arsenite transporter gene (arsB) that indicates the genetic ability of bacteria to tolerate the most toxic arsenic species.

Item Type: Article
Subjects: Environmental Resources, Conservation
Environmental Biotechnology
Depositing User: Dr. S Thul
Date Deposited: 21 May 2019 07:23
Last Modified: 21 May 2019 07:23

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