Seralathan, Muhil Vannan and Devi, S S and Bafana, Amit and Kashyap, S M and Patrizio, A and Krishanamurthi, Kannan (2014) Cytochrome P450 BM3 of Bacillus megaterium - a possible endosulfan biotransforming gene. Journal of Environmental Science, 26 (11). pp. 2307-2314. ISSN 1001-0742

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Computing chemistry was applied to understand biotransformation mechanism of an organochlorine pesticide, endosulfan. The stereo specific metabolic activity of human CYP-2B6 (cytochrome P450) on endosulfan has been well demonstrated. Sequence and structural similarity search revealed that the bacterium Bacillus megaterium encodes CYP-BM3, which is similar to CYP-2B6. The functional similarity was studied at organism level by batch-scale studies and it was proved that B. megaterium could metabolize endosulfan to endosulfan sulfate, as CYP-2B6 does in human system. The gene expression analyses also confirmed the possible role of CYP-BM3 in endosulfan metabolism. Thus, our results show that the protein structure based in-silico approach can help us to understand and identify microbes for remediation strategy development. To the best of our knowledge this is the first report which has extrapolated the bacterial gene for endosulfan biotransformation through in silico prediction approach for metabolic gene identification.

Item Type: Article
Uncontrolled Keywords: CYP-2B6; CYP-BM3; Endosulfan; Protein Structure Similarity
Subjects: Biotechnology
Depositing User: Mr. RK Lohiya
Date Deposited: 02 Aug 2019 05:25
Last Modified: 02 Aug 2019 05:25

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