Ramanan, R and Krishnamurthi, Kannan and Saravana Devi, S and Mudliar, S N and Kaur, S and Tripathi, A K and Chakrabarti, T (2009) Bio-sequestration of carbon dioxide using carbonic anhydrase enzyme purified from Citrobacter freundii. World Journal of Microbiol Biotechnology, 25. pp. 981-987.

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Abstract

The increase in the atmospheric concentrations of one of the vital green house gasses, carbon dioxide, due to anthropogenic interventions has led to several undesirable consequences such as global warming and related changes. In the global effort to combat the predicted disaster, several CO2 capture and storage technologies are being deliberated. One of the most promising biological carbon dioxide sequestration technologies is the enzyme catalyzed carbon dioxide sequestration into bicarbonates which was endeavored in this study with a purified C. freundii SW3 b-carbonic anhydrase (CA). An extensive screening process for biological sequestration using CA has been defined. Six bacteria with high CA activity were screened out of 102 colonies based on plate assay and presence of CA in these bacteria was further emphasized by activity staining and Western blot. The identity of selected bacteria was confirmed by 16S rDNA analysis. CA was purified to homogeneity from C. freundii SW3 by subsequent gel filtration and ion exchange chromatography which resulted in a 24 kDa polypeptide and this is in accordance with the Western blot results. The effect of host on metal ions, cations and anions which influence activity of the enzyme in sequestration studies suggests that mercury and HCO3 - ion almost completely inhibit the enzyme whereas sulfate ion and zinc enhances carbonic anhydrase

Item Type:	Article
Subjects:	Environmental Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 8 not found.
Date Deposited:	22 Mar 2012 07:05
Last Modified:	08 May 2017 06:20
URI:	http://neeri.csircentral.net/id/eprint/159

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