Bafana, Amit (2013) Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii. Carbohydrate polymers, 95 (2). pp. 746-752. ISSN 1879-1344

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Chlamydomonas reinhardtii strain RAC was isolated based on its ability to secrete large amount of exopolysaccharide (EPS). The purified EPS had a molecular weight of 2.25�10(5)Da, and showed fibrillar structure with surfaces having sheet-like appearance. Chemical analysis showed the presence of galacturonic acid, ribose, arabinose, xylose, glucose, galactose and rhamnose sugars. The production of EPS was optimized by the classical one-at-a-time approach and Plackett-Burman design, followed by response surface methodology. The resulting response surface model was statistically significant (p<0.5) and predicted maximum EPS production of 628 mg/L. The optimum production medium consisted of CaCl2 - 74, NaNO3 - 422, K2HPO4 - 10 and MgSO4 - 200mg/L with a pH 7. The EPS showed significant antioxidant activity, which can have several industrial applications. This is the first report on characterization and production of EPS from a Chlamydomonas strain isolated from India. Its differences from the earlier reported EPS are discussed.

Item Type: Article
Uncontrolled Keywords: EPS; Microalgae; Antioxidant; Monosaccharides; Statistical Experimental Design
Subjects: Environmental Biotechnology
Depositing User: Dr Amit Bafana
Date Deposited: 24 Nov 2014 06:24
Last Modified: 24 Nov 2014 06:24

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