Bafana, Amit and Chakrabarti, Tapan and Muthal, Pradeep and Kanade, Gajanan (2009) Detoxification of benzidine-based azo dye by E. gallinarum: time-course study. Ecotoxicology and environmental safety, 72 (3). pp. 960-964. ISSN 1090-2414

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Abstract

Direct black 38 (DB38) dye is a well-established toxic and carcinogenic compound. Present investigation reports isolation of an Enterococcus gallinarum strain capable of decolorizing and degrading it. Changes in toxicity and mutagenicity of DB38 and its metabolites were also determined using a battery of carefully selected tests (cytotoxicity, respiration inhibition test and Ames test). Toxicity assays were carried out on E. gallinarum itself as this also gave information about suitability of this strain for the dye decolorization operation. The strain was found to reduce both toxicity and mutagenicity of DB38 metabolites. Benzidine and 4-aminobiphenyl (4-ABP) were identified as the DB38 metabolites, responsible for its toxic and mutagenic properties, by HPLC-MS analysis. Further degradation of benzidine and 4-ABP was found to result in the decrease in toxicity and mutagenicity.

Item Type:	Article
Uncontrolled Keywords:	Toxicity; Mutagenicity; Amestest; Azodye; Benzidine;4-Aminobiphenyl
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Dr Amit Bafana
Date Deposited:	24 Nov 2014 06:01
Last Modified:	24 Nov 2014 06:01
URI:	http://neeri.csircentral.net/id/eprint/330

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