Bafana, Amit and Jain, Minakshi and Agrawal, Gaurav and Chakrabarti, T (2009) Bacterial reduction in genotoxicity of Direct Red 28 dye. Chemosphere, 74 (10). pp. 1404-6. ISSN 1879-1298

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Abstract

Direct Red 28 (DR28) is a benzidine-based azo dye widely used in several countries. It has also been a subject of intense research for its anti-prion activity. Like other benzidine-based azo dyes, it is also carcinogenic and toxic. However, there are very few studies addressing its detoxification. In the present study, a Bacillus velezensis strain was used for detoxification of DR28. Toxicity was checked by a battery of highly sensitive genotoxicity assays like comet assay, DNA ladder formation, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay and flow cytometric Annexin V binding assay. HL-60 cell line was used as the test system. All the assays showed an initial increase in toxicity upon biodegradation due to release of mutagenic products, like benzidine and 4-aminobiphenyl, from the dye. These intermediates caused significant DNA damage and induced apoptosis in HL-60 cells. Then the culture degraded these mutagenic intermediates, due to which the toxicity was reduced gradually, finally resulting in nearly complete detoxification.

Item Type:	Article
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Dr Amit Bafana
Date Deposited:	25 Sep 2013 07:24
Last Modified:	25 Jul 2014 09:53
URI:	http://neeri.csircentral.net/id/eprint/334

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