Soni, Deepika and Bafana, Amit and Gandhi, Deepa and Saravana Devi, S and Pandey, R A (2014) Stress Response of Pseudomonas Species to Silver Nanoparticles at the Molecular Level. Environmental Toxicology and Chemistry, 33 (9). pp. 2126-2132. ISSN 0730-7268

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In recent years, silver nanoparticles (AgNPs) have been shown to possess broad antibacterial activity. The present study investigated the cytotoxicity of AgNPs to a common soil bacterium, Pseudomonas sp. The molecular mechanism involved in its stress response to AgNPs was also studied. The minimum inhibitory concentration (MIC) of AgNPs was found to be 0.2 mg/L. At a sublethal concentration of 0.1 mg/L AgNPs, the protein expression profile of Pseudomonas showed overexpression of stress proteins such as ribosomal proteins S2 and L9, alkyl hydroperoxide reductase/thiol-specific antioxidant (AhpC/TSA) family protein, and ketohydroxyglutarate aldolase (KHGA). The upregulation of these proteins was further confirmed by quantitative polymerase chain reaction. The results showed increased expression of ribosomal protein S2, KHGA, AhpC/TSA, and ribosomal protein L9 by 1.09-, 3.41-, 1.52-, and 1.56-fold, respectively (p < 0.05), after AgNP exposure compared with control. The present study clearly demonstrates that AgNPs are toxic to soil bacteria and induce oxidative and metabolic stress.

Item Type: Article
Uncontrolled Keywords: Stress Response; Nanoparticles; Nanotoxicology; Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry; Molecular Mechanism
Subjects: Environmental Health
Depositing User: Pravin Krishnarao Naoghare
Date Deposited: 28 Apr 2016 06:27
Last Modified: 18 Jul 2019 10:07

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