Minami　, Y and Sarangi, B K and Pattanaik, L and Mishra, A and Bakde, R and Thul, S T (2015) Cell cultures and molecular investigation on Polygonum tinctorium and Indigofera tinctoria plants to understand indican biosynthesis. In: 56th Annual Meeting of the Japanese Society for Plant Physiologists, March 16-18, 2015, Tokyo University of Agriculture (TUA), Tokyo, Japan .

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Abstract

Indican biosynthesis in Indigofera tinctoria and Polygonum tinctorium plants is of topical interest due to its academic and industrial relevance for indigo dye production. Dye yield depends on indican content in the plant biomass. Cell culture and molecular biological investigations were carried out to assess indican biosynthesis in these plants. Tissue culture protocols were optimized for explant identification, decontamination, in vitro culture medium & suitable growth regulators and culture conditions for P. tinctorium at Okayama University of Science, and I. tinctoria at CSIR-NEERI. Four different growth hormones i.e. BA, Kin, NAA, 2,4-D, at 0.01 –2 mg L-1 culture medium, in random combination, and two different explants i.e. leaf and internode of both plants were experimented. In both plants, callus proliferation was better from leaf tissue with growth index (GI) up to 10 on MS agar gelled medium fortified with BA+NAA in comparison to BA+2,4-D. Suspension cell cultures of I. tinctorium were induced in MS liquid medium with only 2,4-D through 3 stages with GI up to 30. In vitro raised cell biomass of I. tinctorium presented higher indican synthesis (p> 0.5) in comparison to that of P. tinctorium. Both of these plants synthesize indican, but the differential response under in vitro is interesting. Total transcriptomes of both plants were worked out and annotated. Comparative analysis of transcriptome profile indicated >80% genes are similar for the indican biosynthetic pathways. Complete alignment of both transcriptomes and validation for biosynthesis pathways specific genes is needed in both the plants to ascertain their differential expression.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Environmental Biotechnology Biochemistry Botany
Divisions:	UNSPECIFIED
Depositing User:	Dr. S Thul
Date Deposited:	12 Jan 2017 09:40
Last Modified:	12 Jan 2017 09:40
URI:	http://neeri.csircentral.net/id/eprint/647

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