Bhagchandani, Sharda P and Kubade, Sushant and Nikhare, Priyanka P and Manke, Sonali and Chandak, Nitin H and Kabra, Dinesh and Baheti, Neeraj N and Agrawa, Vijay S and Sarda, Pankaj and Mahajan, Parikshit and Ganjre, Ashish and Purohit, H J and Singh, Lokendra and Taori, Girdhar M and Daginawala, Hatim F and Kashyap, Rajpal S (2016) Nested PCR Assay for Eight Pathogens: A Rapid Tool for Diagnosis of Bacterial Meningitis. Molecular Diagnosis and Therapy, 20 (1). pp. 45-54. ISSN 1177-1062, ESSN: 1179-2000

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Abstract

Purpose Bacterial meningitis is a dreadful infectious disease with a high mortality and morbidity if remained undiagnosed. Traditional diagnostic methods for bacterial meningitis pose a challenge in accurate identification of pathogen, making prognosis difficult. The present study is therefore aimed to design and evaluate a specific and sensitive nested 16S rDNA genus-based polymerase chain reaction (PCR) assay using clinical cerebrospinal fluid (CSF) for rapid diagnosis of eight pathogens causing the disease. Methods The present work was dedicated to development of an in-house genus specific 16S rDNA nested PCR covering pathogens of eight genera responsible for causing bacterial meningitis using newly designed as well as literature based primers for respective genus. A total 150 suspected meningitis CSF obtained from the patients admitted to Central India Institute of Medical Sciences (CIIMS), India during the period from August 2011 to May 2014, were used to evaluate clinical sensitivity and clinical specificity of optimized PCR assays. Results The analytical sensitivity and specificity of our newly designed genus-specific 16S rDNA PCR were found to be C92 %. With such a high sensitivity and specificity, our in-house nested PCR was able to give 100 % sensitivity in clinically confirmed positive cases and 100 % specificity in clinically confirmed negative cases indicating its applicability in clinical diagnosis.

Item Type:	Article
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Dr. H J Purohit
Date Deposited:	03 Apr 2017 07:04
Last Modified:	03 Apr 2017 07:04
URI:	http://neeri.csircentral.net/id/eprint/814

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