Shaligram, Shraddha and Kumbhare, Shreyas V and Dhotre, Dhiraj P and Muddeshwar, Manohar G and Kapley, Atya and Joseph, Neetha and Purohit, H P and Shouche, Yogesh S and Pawar, Shrikant P (2016) Genomic and functional features of the biosurfactant producing Bacillus sp. AM13. Functional & Integrative Genomics, 16 (5). pp. 557-566. ISSN 1438-793X, ESSN: 1438-7948

[img] PDF - Published Version
Restricted to Registered users only

Download (1393Kb) | Request a copy


Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillussp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.

Item Type: Article
Uncontrolled Keywords: Biosurfactant; Lipopeptides; Bacillus Genome; NRPS; Bacterial Natural Products
Subjects: Microbiology
Depositing User: Dr. H J Purohit
Date Deposited: 03 Apr 2017 06:54
Last Modified: 03 Apr 2017 06:54

Actions (login required)

View Item View Item