Shekhawata, Seema D and Purohit, H J and Taoria, Girdhar M and Daginawala, Hatim F and Kashyap, Rajpal S (2016) Evaluation of heat shock proteins fordiscriminating between latenttuberculosis infection and activetuberculosis: A preliminary report. Journal of Infection and Public Health, 9 (2). pp. 143-152. ISSN 1876-0341, ESSN: 1876-035X

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The diagnosis of a latent tuberculosis infection (LTBI) is of the utmost concern. The available tests, the tuberculin skin test (TST) and the QuantiferonTB Gold test (QFT-G) cannot discriminate between active TB and LTBI. Therefore, the aim of the study is to identify new biomarkers that can discriminate between active TB and LTBI and can also assess the risk of the individual developing active TB. In total, 55 blood samples were collected, of which 10 samples were from the active TB infection group, 10 were from the high-risk exposure group, 23 were from the low-risk exposure group, and 12 were from healthy controls living in a non-TB endemic area. A panel of heat shock proteins (Hsps), including host Hsp25, Hsp60, Hsp70, and Hsp90 and Mycobacterium tuberculosis (MTB) Hsp16, were evaluated in all of the collected samples using ELISA. The levels of the host Hsp(s) (Hsp25, Hsp60, Hsp70 and Hsp90) and MTB Hsp16 were significantly (p < 0.05) elevated in the active TB group compared to the high-risk exposure group, the low-risk exposure group and the control group. Notably, the levels of the same panel of Hsp(s) were elevated in the high-risk exposure group compared to the low-risk exposure group. On follow-up, out of the 10 high-risk exposure participants, 3 converted into active TB, indicating that this group has the highest risk of developing TB. Thus, the evaluated panel of Hsp(s) can discriminate between LTBI and active TB. They can also identify individuals who are at the highest risk of developing active TB. Because they can be rapidly detected, Hsp(s) have an edge over the existing diagnostic tools for LTBI. The evaluation of these proteins will be useful in designing better diagnostic methods for LTBI.

Item Type: Article
Uncontrolled Keywords: Tuberculosis; Latent Tuberculosis; Infection; Heat Shock Proteins; Biomarkers; Mycobacterium tuberculosis; Diagnosis
Subjects: Environmental Health
Depositing User: Dr. H J Purohit
Date Deposited: 02 Apr 2017 16:56
Last Modified: 02 Apr 2017 16:56

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