Nagdev, Khushboo J and Bhagchandani, Sharda P and Bhullar, Shradha S and Kapgate, Rajkumar C and Kashyap, Rajpal S and Chandak, Nitin H and Daginawala, Hatim F and Purohit, H J and Taori, Girdhar M (2015) Rapid Diagnosis and Simultaneous Identification of Tuberculous and Bacterial Meningitis by a Newly Developed Duplex Polymerase Chain Reaction. Indian Journal of Microbiology, 55 (2). pp. 213-218. ISSN 0046-8991, ESSN: 0973-7715

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The present study describes the development and evaluation of a duplex polymerase chain reaction (DPCR) for diagnosis and simultaneous identification of tuberculous meningitis (TBM) and bacterial meningitis (BM) in a single reaction. A D-PCR with primers amplifying portions of the Mycobacterium tuberculosis IS6110 and the eubacteria 16SrDNA sequence in a same reaction mix was developed and tested on DNA extracted from 150 clinical CSF samples from different categories (TBM = 39, BM = 26, control infectious and non-infectious category = 85). The results indicate a clear differentiation between bands for eubacteria and M. tuberculosis with an analytical sensitivity of 103 cfu/ml for eubacteria and 102 cfu/ml for M. tuberculosis. When evaluated in clinical samples, D-PCR overall diagnosed 100 % confirmed TBM and 100 % confirmed BM cases with overall specificity of 96.5 %. D-PCR can be an effective tool for diagnosis and simultaneous identification of TBM or BM in a single PCR reaction. It saves time, cost, labour and sample amount and help in administration of appropriate antimicrobial therapy.

Item Type: Article
Uncontrolled Keywords: Bacterial Meningitis; Tuberculous Meningitis; Diagnosis; Duplex PCRI
Subjects: Microbiology
Depositing User: Dr. H J Purohit
Date Deposited: 01 Apr 2017 09:08
Last Modified: 01 Apr 2017 09:08

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