Aloni , Prutha D and Nayak, Amit R and Chaurasia, Sweta R and Deopujari, Jayant Y and Chourasia , Chhaya and Purohit, H J and Taori, Girdhar M and Daginawala , Hatim F and Kashyap, Rajpal S (2016) Effect of Fagonia arabica on thrombin induced release of t-PA and complex of PAI-1 tPA in cultured HUVE cells. Journal of Traditional and Complementary Medicine, 6 (3). pp. 219-223. ISSN 2225-4110

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Fagonia arabica (FA) possesses a thrombolytic property which has been earlier reported in our laboratory. Current study was undertaken to investigate the effect of aqueous extract of FA on thrombin-induced tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) release from cultured human umbilical vein endothelial cell line (HUVE) for studying its clot lytic activity. For this, establishment of cell line model has been done by isolating the cells from human umbilical cord. Cell toxicity was evaluated using XTT assay. Estimation of t-PA and PAI-1 t-PA complex were done using ELISA technique. Thrombin treatment induces the t-PA and PAI-1 release from HUVE cell line, and FA treatment was found to antagonize the thrombin induced t-PA and PAI-1 release. Our preliminary results suggest that FA may be used as an alternative to thrombolytic drug. However, study demands further experiments using animal model of thrombosis to establish the role of FA as a novel thrombolytic drug. Copyright © 2015, Center for Food and Biomolecules, National Taiwan University. Production and hosting

Item Type: Article
Uncontrolled Keywords: HUVE Cell Line; Fagonia Arabica (FA); Thrombin; Tissue Plasminogen Activator (t-PA); Plasminogen Activator Inhibitor-1 (PAI-1)
Subjects: Microbiology
Depositing User: Dr. H J Purohit
Date Deposited: 01 Apr 2017 08:49
Last Modified: 01 Apr 2017 08:49

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