Khairnar, Krishna and Lynde, Carrie and Keystone , Jay and Ralevski, Filip and McCarthy , Anne and Levine , Jeremy and Pillai, Dylan R. (2016) Molecular diagnosis of leishmaniasis at the subgenus and species level. Parasitology Research, 115 (1). p. 49. ISSN 0932-0113, ESSN: 1432-1955

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We report three cases of imported cutaneous leishmaniasis in Canada. The current diagnostic modalities such as culture and histopathology demand expertise and are time consuming. We adapted a rapid molecular diagnostic method like real-time PCR (RT-PCR) for leishmaniasis, which enabled speciation not possible by traditional culture methods. Based on the findings of RT-PCR, the management of cutaneous leishmaniasis caused by Leishmania (Leishmania ) tropica may be done with a conservative approach; an aggressive treatment approach may be adapted for management of cutaneous leishmaniasis caused by Leishmania (Viannia) panamensis especially considering the potential risk of Leishmania Viannia complex causing mucocutaneous leishmaniasis. The RT-PCR assay described here enables the speciation of Leishmania, which may be useful to the clinical management of leishmaniasis in returning travelers.

Item Type: Article
Subjects: Microbiology
Depositing User: Dr Krishna Khairnar
Date Deposited: 05 Apr 2017 11:30
Last Modified: 05 Apr 2017 11:30

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