Shrivastava , Preeti and Neoghare, Pravin and Gandhi, Deepa and Devi, Saravana and Krishnamurthi, Kannan and Bafana, Amit and Kashyap, Sanjay and Chakrabarti, Tapan (2017) Application of cell-based assays for toxicity characterization of complex wastewater matrices: Possible applications in wastewater recycle and reuse. Ecotoxicology and Environmental Safety, 142 (1). pp. 60-71. ISSN 0147-6513

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Exposure to pre-concentrated inlet or outlet STP wastewater extracts at different concentrations (0.001% to 1%) induced dose-dependent toxicity in MCF-7 cells, whereas drinking water extracts did not induce cytotoxicity in cells treated. GC-MS analysis revealed the occurrence of xenobiotic compounds (Benzene, Phthalate, etc.) in inlet/outlet wastewater extracts. Cells exposed to inlet/outlet extract showed elevated levels of reactive oxygen species (ROS: inlet: 186.58%, p<0.05, outlet, 147.8%, p<0.01) and loss of mitochondrial membrane potential (Δψm: inlet, 74.91%, p<0.01; outlet, 86.70%, p<0.05) compared to the control. These concentrations induced DNA damage (Tail length: inlet: 34.4%, p<0.05, outlet, 26.7%, p<0.05) in treated cells compared to the control (Tail length: 7.5%). Cell cycle analysis displayed drastic reduction in the G1 phase in treated cells (inlet, G1:45.0%; outlet, G1:58.3%) compared to the control (G1:67.3%). Treated cells showed 45.18% and 28.0% apoptosis compared to the control (1.2%). Drinking water extracts did not show any significant alterations with respect to ROS, Δψm, DNA damage, cell cycle and apoptosis compared to the control. Genes involved in cell cycle and apoptosis were found to be differentially expressed in cells exposed to inlet/outlet extracts. Herein, we propose cell-based toxicity assays to evaluate the efficacies of wastewater treatment and recycling processes.

Item Type: Article
Uncontrolled Keywords: Cell-Based Assays; Recycling and Reuse; Mitochondrial Membrane Potential; Cell cycle Reactive oxygen species Apoptosis
Subjects: Environmental Health
Depositing User: Mr S M Kashyap
Date Deposited: 27 Apr 2017 06:34
Last Modified: 18 Jul 2019 09:19

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