Dhondge, Alka and Surendran, Subin and Seralathan, Muhil Vannan and Naoghare, Pravin K and Krishnamurthi, Kannan and Saravana Devi, Sivanesan and Chakrabarti, Tapan (2012) Cellular alterations and modulation of protein expression in bitumen-challenged human osteoblast cells. Environmental Science and Pollution Research, 19 (9). pp. 4030-4041. ISSN 0944-1344, ESSN: 1614-7499

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Abstract

Purpose There are many arguments on the carcinogenic potential of bitumen extract. The mechanism of bitumen-induced damage is not well understood at the molecular level. Therefore, in the present study, cell-transforming and tumor-inducing potential of bitumen extract was studied using in vitro [human osteosarcoma (HOS) cells] and in vivo [nude and severe combined immunodeficiency (SCID) mice] models. Methods Gas chromatography/mass spectrometry (GC/MS) analysis was carried out to find out the existence of carcinogenic compounds in the bitumen extract. Cell transformation test, anchorage independence assay, karyotyping assay, tumorigenicity assay, and 2-DE analysis were used to find out the effect of bitumen using the in vitro and in vivo models. Results GC/MS analysis showed the existence of carcinogenic compounds in the bitumen extract. HOS cells were treated with different concentrations (25, 50, and 100 μl/ml) of bitumen extract. Compared to the parental HOS cells, bitumen transformants (HOS T1 and HOS T2) showed the characteristics of anchorage independency, chromosomal anomaly, and cellular transformation. Interestingly, bitumen transformants were not able to form tumor in nude/SCID mice. Proteomic analysis revealed the existence of 19 differentially expressed proteins involved in progression of cancer, angiogenesis, cell adhesion, etc. Conclusions Exposure of bitumen extract to HOS cells results in the cellular transformation similar to cancer cells and can modulate proteins involved in the progression of cancer. We state that the non-tumorogenic potential of bitumen transformant in nude/SCID mice can be attributed to the downregulation of galectin-1, chromodomain helicase DNA-binding protein 1-like gene, and membrane-associated guanylate kinase 2 protein.

Item Type:	Article
Uncontrolled Keywords:	Human Osteosarcoma Cell; Karyotyping Assay; Bitumen Extract; Nude Mice; SCID Mice; Cellular Transformation
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Dr Krishnamurthi Kannan
Date Deposited:	09 May 2017 08:56
Last Modified:	09 May 2017 08:56
URI:	http://neeri.csircentral.net/id/eprint/922

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