Wanjari, Snehal and Prabhu, Chandan and Yadav, Renu and Satyanarayana, T and Labhsetwar, Nitin and Rayalu, Sadhana (2011) Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction. Process Biochemistry, 46. pp. 1010-1018. ISSN 1359-5113

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Abstract

Carbonic anhydrase (CA) has been immobilized on chitosan beads and has been tested for its activity using p-NPA. CA immobilized chitosan beads were further tested for targeted application of carbonation reaction to convert CO2 to CaCO3. It was observed that onset time for precipitation of CaCO3 was 40 s for immobilized catalyst as compared to non-catalyzed system (100 s). Mechanistic and kinetic aspects have been addressed for immobilized catalyst as well as free CA using p-NPA assay. Km and Vmax of the immobilized CA was observed to be 2.36mM and 0.54�moles/min/ml as compared to Km and Vmax of 0.87mMand 0.93�moles/min, respectively, for free CA. Storage stability test was conducted up to 20 days and it was observed that at −20 ◦C, the Half Life Period (HLP) of immobilized CA was 216 h as compared to 192 h for free CA whereas at 25 ◦C the HLP for immobilized and free CA was observed to be 456 h and 408 h, respectively.

Item Type:	Article
Uncontrolled Keywords:	Chitosan Beads; Carbonic Anhydrase; Immobilization; Carbonation Reaction; Kinetics; Stability
Subjects:	Materials Science
Divisions:	UNSPECIFIED
Depositing User:	Dr. Nitin Labhasetwar
Date Deposited:	10 Jan 2018 05:27
Last Modified:	10 Jan 2018 05:27
URI:	http://neeri.csircentral.net/id/eprint/1036

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