Sain, Soumyadeep and Naoghare, P and Saravana Devi , S and Daiwile, Atul and Krishnamurthi, Kannan and Arrigo, Patrizio and Chakrabarti, Tapan (2014) Beta caryophyllene and caryophyllene oxide, isolated from Aegle marmelos, as the potent anti-inflammatory agents against lymphoma and neuroblastoma cells. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry, 13 (1). pp. 44-55. ISSN 1871-5230

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Abstract

Aegle Marmelos (Indian Bael) is a tree belonging to the family of Rutaceae. It holds a prominent position in both Indian medicine and culture. We have screened various fractions of Aegle marmelos extracts for its anti-cancer properties using in vitro cell models. Gas chromatography-Mass spectrometry (GC-MS) was employed to analyze the biomolecules present in the Aegle marmelos extract. Jurkat and human neuroblastoma (IMR-32) cells were treated with different concentrations of the fractionated Aegle marmelos extracts. Flow cytometric analysis revealed that optimal concentration (170µg/ml) of beta caryophyllene and caryophyllene oxide fractions of Aegle marmelos extract can induce apoptosis in Jurkat cell line. cDNA expression profiling of pro-apoptotic and anti-apoptotic genes was carried out using real time PCR (RT-PCR). Down-regulation of anti-apoptotic genes (bcl-2, mdm2, cox2 and cmyb) and up-regulation of pro-apoptotic genes (bax, bak1, caspase-8, caspase-9 and ATM) in Jurkat and IMR-32 cells treated with the beta caryophyllene and caryophyllene oxide fractions of Aegle marmelos extract revealed the insights of the downstream apoptotic mechanism. Furthermore, in-silico approach was employed to understand the upstream target involved in the induction of apoptosis by the beta caryophyllene and caryophyllene oxide fractions of Aegle marmelos extract. Herein, we report that beta caryophyllene and caryophyllene oxide isolated from Aegle marmelos can act as the potent anti-inflammatory agents and modulators of a newly established therapeutic target, 15-lypoxygenase (15-LOX). Beta caryophyllene and caryophyllene oxide can induce apoptosis in lymphoma and neuroblastoma cells via modulation of 15-LOX (up-stream target) followed by the down-regulation of anti-apoptotic and up-regulation of pro-apoptotic genes.

Item Type:	Article
Uncontrolled Keywords:	Apoptotic Genes; Caryophyllene; 15-lipoxygenase; Lymphoma; Neuroblastoma Cells
Subjects:	Environmental Health Biochemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr Krishnamurthi Kannan
Date Deposited:	24 Nov 2014 06:34
Last Modified:	28 Apr 2016 06:31
URI:	http://neeri.csircentral.net/id/eprint/307

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