Qureshi, Asifa and Kapley, A and Purohit, H J (2012) Degradation of 2,4,6-Trinitrophenol (TNP) by Arthrobacter sp.HPC1223 Isolated from Effluent Treatment Plant. Indian Journal of Microbiology, 52 (4). pp. 642-647. ISSN 0046-8991

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Abstract

Arthrobacter sp. HPC1223 (Genebank Accession No. AY948280) isolated from activated biomass of effluent treatment plant was capable of utilizing 2,4,6 trinitrophenol (TNP) under aerobic condition at 30 �C and pH 7 as nitrogen source. It was observed that the isolated bacteria utilized TNP up to 70 % (1 mM) in R2A media with nitrite release. The culture growth media changed into orange-red color hydride-meisenheimer complex at 24 h as detected by HPLC. Oxygen uptake of Arthrobacter HPC1223 towards various nitro/amino substituted phenols such as dinitrophenol(1.2 nmol/min/mg cells), paranitrophenol (0.9 nmol/min/mg cells), 2-aminophenol (0.75 nmol/min/mg cells), p-aminophenol (0.4 nmol/min/mg cells), phenol (0.56 nmol/min/mg cells) and TNP (2.42 nmol/min/mg cell) was analysed, which showed its additional characteristic of broad substrate catabolic capacity. The present study thus report a novel indigenous bacteria isolated from activated sludge utilized TNP and has broad catabolic potential towards substituted phenols.

Item Type: Article
Uncontrolled Keywords: Trinitrophenol;Arthrobacter;ETP;Nitrite;Degradation
Subjects: Environmental Pollution
Microbiology
Divisions: UNSPECIFIED
Depositing User: Dr ASIFA QURESHI
Date Deposited: 07 Mar 2014 04:59
Last Modified: 07 Mar 2014 04:59
URI: http://neeri.csircentral.net/id/eprint/428

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