Gandhi, Deepa and Tarale, Prashant and Naoghare, Pravin and Bafana, Amit and Krishnamurthi, Kannan and Arrigo, Patrizio and Saravana Devi, S (2015) An integrated genomic and proteomic approach to identify signatures of endosulfan exposure in hepatocellular carcinoma cells. Pesticide Biochemistry and Physiology, 125. pp. 8-16. ISSN 0048-3575

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Abstract

Present study reports the identi fication of genomic and proteomic signatures of endosulfan exposure in hepatocellular carcinoma cells (HepG2). HepG2 cells were exposed to sublethal concentration (15 μM) of endosulfan for 24 h. DNA microarray and MALDI –TOF–MS analyses revealed that endosulfan induced signi ficant alterations in the expression level of genes and proteins involved in multiple cellular pathways (apoptosis, transcription, immune/in flammatory response, carbohydrate metabolism, etc.). Furthermore, downregulation of PHLDA gene, upregulation of ACIN1 protein and caspase-3 activation in exposed cells indicated that endosulfan can trigger apoptotic cascade in hepatocellular carcinoma cells. In total 135 transcripts and 19 proteins were differentially expressed. This study presents an integrated approach to identify the alteration of biological/cellular pathways in HepG2 cells upon endosulfan exposure

Item Type:	Article
Uncontrolled Keywords:	Endosulfan; Hepatocellular Carcinoma Cells (HepG2); Gene Microarray; Proteomics; Common Signatures
Subjects:	Environmental Health
Divisions:	UNSPECIFIED
Depositing User:	Pravin Krishnarao Naoghare
Date Deposited:	28 Apr 2016 09:41
Last Modified:	28 Apr 2016 09:42
URI:	http://neeri.csircentral.net/id/eprint/572

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