Husain, Aliabbas A and Daginawala, Hatim F and Warke, Shubangi R and Kalorey, Devanand R and Kurkure, Nitin V and Purohit, H J and Taori, Girdhar M and Kashyap, Rajpal S (2015) Investigation of Immune Biomarkers Using Subcutaneous Model of M. tuberculosis Infection in BALB/c Mice: A Preliminary Report. Immune Network, 15 (2). pp. 83-90. ISSN 1598-2629, ESSN: 2092-6685

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Abstract

Evaluation and screening of vaccines against tuberculosis depends on development of proper cost effective disease models along with identification of different immune markers that can be used as surrogate endpoints of protection in preclinical and clinical studies. The objective of the present study was therefore evaluation of subcutaneous model of M.tuberculosis infection along with investigation of different immune biomarkers of tuberculosis infection in BALB/c mice. Groups of mice were infected subcutaneously with two different doses : high (2×106 CFU) and low doses (2×102 CFU) of M.tuberculosis and immune markers including humoral and cellular markers were evaluated 30 days post M.tuberculosis infections. Based on results, we found that high dose of subcutaneous infection produced chronic disease with significant (p<0.001) production of immune markers of infection like IFNγ, heat shock antigens (65, 71) and antibody titres against panel of M.tuberculosis antigens (ESAT-6, CFP-10, Ag85B, 45kDa, GroES, Hsp-16) all of which correlated with high bacterial burden in lungs and spleen. To conclude high dose of subcutaneous infection produces chronic TB infection in mice and can be used as convenient alternative to aerosol models in resource limited settings. Moreover assessment of immune markers namely mycobacterial antigens and antibodies can provide us valuable insights on modulation of immune response post infection. However further investigations along with optimization of study protocols are needed to justify the outcome of present study and establish such markers as surrogate endpoints of vaccine protection in preclinical and clinical studies in future

Item Type:	Article
Uncontrolled Keywords:	Mice; Tuberculosis; Subcutaneous Route; Immune Markers
Subjects:	Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Dr. H J Purohit
Date Deposited:	02 Apr 2017 16:12
Last Modified:	02 Apr 2017 16:12
URI:	http://neeri.csircentral.net/id/eprint/819

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