Ramkumar, K M and Sankar, L and Manjula, C and Krishnamurthi, Kannan and Devi, S S and Chakrabarti, T and Kalaiselvi, K and Palanivel, M and Rajaguru, P (2010) Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line. Environmental and Molecular Mutagenesis, 51 (4). pp. 285-293. ISSN 0893-6692, ESSN: 1098-2280

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Abstract

In this study we have evaluated the genoprotective effect of the ethanol extract of Gymnema montanum (GLEt) leaves in human peripheral blood lymphocytes and HL-60 cell line in vitro using the comet assay. DNA damage was induced by treating the cells with H2O2 and methyl methane sulphonate (MMS). GLEt treatment effectively protected the lymphocytes and HL-60 cell line from H2O2-induced oxidative DNA damage in a dosedependent manner whereas it was not effective against alkylative DNA damage caused by MMS. The global percent repair efficiency also showed that both pre- and post- GLEt treatment provided effective protection against H2O2 induced DNA damage but not as effective against MMS. At 200 lg ml21 level, its repair capacity against H2O2 induced DNA damage was comparable to that of vitamin-C (100 lM). Furthermore, exposure to GLEt reduced the formation of apoptotic cells caused by H2O2, which was demonstrated by the decreased sub-G1-DNA content in cell cycle analysis and apoptotic frequencies of lymphocytes in an annexin-V binding assay. In addition, GLEt was found to have effective peroxide scavenging ability in dose-dependent manner. The protective efficiency of the extract was found to be directly proportional to its total phenolic content. The present study indicates that G. montanum leaves are a significant source of phytochemicals with antigenotoxic and antioxidant activity, and thus has potential therapeutic use. Environ. Mol. Mutagen

Item Type:	Article
Uncontrolled Keywords:	Comet Assay; Human Lymphocytes; Gymnema Montanum; Genotoxicity; Total Phenolics; HL-60 Cell Line
Subjects:	Biochemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr Krishnamurthi Kannan
Date Deposited:	09 May 2017 09:06
Last Modified:	09 May 2017 09:06
URI:	http://neeri.csircentral.net/id/eprint/935

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